Journal: Genomics Data

Article Title: RNAome sequencing delineates the complete RNA landscape

doi: 10.1016/j.gdata.2015.07.002

Figure Lengend Snippet: Schematic of the Total RNA Analysis Pipeline, TRAP for analysis of sequencing datasets. (A) Modules for long RNA analysis, script 1 (green) for RefSeq annotated exonic transcripts and script 2 (red) for RefSeq annotated non-exonic regions. (B) Modules for small RNA analysis, script 3 (blue) to sequentially align trimmed reads to rRNA, tRNA fragments and the microRNA database (miRBase version 21).

Article Snippet: These samples were used for RNAome sequencing as well as standard poly(A)+-sequencing (Illumina TruSeq mRNA), standard small RNA sequencing (Illumina TruSeq smallRNA v1.5) and Affymetrix gene expression arrays (HT Mouse Genome 430 PM), the latter three methods all serve as controls for RNAome sequencing and were all performed according to manufacturer's protocol.

Techniques: Sequencing

Journal: Genomics Data

Article Title: RNAome sequencing delineates the complete RNA landscape

doi: 10.1016/j.gdata.2015.07.002

Figure Lengend Snippet:

Article Snippet: These samples were used for RNAome sequencing as well as standard poly(A)+-sequencing (Illumina TruSeq mRNA), standard small RNA sequencing (Illumina TruSeq smallRNA v1.5) and Affymetrix gene expression arrays (HT Mouse Genome 430 PM), the latter three methods all serve as controls for RNAome sequencing and were all performed according to manufacturer's protocol.

Techniques: RNA Sequencing, Comparison

Journal: Molecular Cell

Article Title: METTL1 Promotes let-7 MicroRNA Processing via m7G Methylation

doi: 10.1016/j.molcel.2019.03.040

Figure Lengend Snippet:

Article Snippet: NEBNext SmallRNA kit , NEB , Cat# E7300S.

Techniques: Recombinant, Protease Inhibitor, Expressing, Luciferase, Labeling, RNA HS Assay, Microarray, Imaging, Software, Autoradiography, Bradford Assay, Modification, Migration